[So I have decided to grow my own phytoplankton culture so that I can dose it to my reef and have as food for future rotifer culture to feed fish fry.][Thought I would share some details of my setup and what I did.][So for containers, I am using the same setup as my reef dosing containers, two of the 2.5 Qt. OXO glass FlipLock Storage Containers.][I drilled a hole in the top to add a 1/4" Push Connect Bulkhead Tank Adapter into the lid with rigid tubing inside, to the bottom and a stem/barbed elbow on top. I also drilled a very small hole in the top for venting.][I wanted something self-contained, secure and portable in case I need to move it anywhere. (Running out of room in my house for fish stuff

)][So I bought a tote from Walmart to house my containers and equipment as well as conceal most of the lighting. Luckily the tote was a perfect fit for this project. There was a perfect groove in the bottom for me to make and fit a small 2x4 wood frame to keep the bottom of the containers secure and close together. I also screwed another piece of 1x4 to the frame to act as a mount for the light to slip over. ][
View attachment 1491]
View attachment 1492View attachment 1493[The light I am using is a “Warmoon Outdoor LED Flood Light, 10W Daylight White 6500K Waterproof Security Light” on Amazon. I cut a small hole in the back of the tote, close to the bottom, for the cord to come out. ][Then I marked and cut out the tote lid so the containers could also stick out the top and also secure the tops. So now the containers do not move inside the tote and are secure.][
View attachment 1494][I then measured, marked and labeled my containers at the 1 and 2 liter mark. Using a cheap dual outlet pump from Walmart, I hooked up some tubing, filled with water and tested. It worked great! The tubing I am using is just expired, still sterile in the package, IV tubing with roller clamp. They just get thrown away so I have a huge bag full of them ][J][…I don’t like the gang valves and this tubing is very small. ][
View attachment 1495][After testing, I put about a cup of rubbing alcohol in each one and shook real well, emptied and set to air dry to sterilize. ][I ordered the algae culture disks from Florida Aqua Farms for this culture. I did the recommended instructions and put a little sterile RODI water on the disk and let set for 24 hrs. Then I used the sterile swab provided and rubbed the swab all around to remove the algae from the disk and mix with water on the disk giving me just a little green “soup”. Next I filled one of my containers with 2 liters of 1.019 saltwater, added the green algae soup from the disk and added 5 ml of Miracle Grow. I put the container in the tote with the light running for 16 hours and connected the air to bubble at max 24/7. ][I stir the container every night and so far the results are looking good! Here are the results so far.][
View attachment 1496]After about 7-9 days I will split the culture in half between the 2 containers, fill both back to the 2 liter mark with saltwater and add 5 ml of Miracle Grow to each and keep stirring every day and just keep on with the cycle.